AAI Immunology 2019 Poster
Francisco J. Martinez-Becerra, Roddy S. O’Connor, Saba Ghassemi, David Heo & Alyssa M. Master
The field of cell therapy is shifting away from animal origin components used in the culture or preservation of cells and replacing them with human or chemically defined components. However, this shift often leads to diminished cell proliferation and functionality due to the lack of physiologically relevant proteins. Therefore, there is an important need for generation of media supplements to optimize cell therapy.
Physiologix™ XF Human Growth Factor Concentrate (hGFC) is a cGMP, xeno-free media supplement that can be used as a replacement to supplements such as human serum. In this study, T cells were isolated from blood and cultured for 14 days in OpTmizer or X-VIVO 15 supplemented with 5% human serum or 2% Physiologix™ XF. Population doublings and cell size were defined, as well as CD4/CD8 and memory markers (CCR7 and CD45RO) to assess phenotype. We detected an increase in naïve and central memory populations in 2% Physiologix™ XF compared to human serum across all tested basal media. Higher transduction efficiency was observed when using 2% Physiologix™ XF at a high MOI of 4 and was conserved across all dilutions including a MOI of <1